为什么western blot 的条带有很多非特异性条带？ 有可能是一抗的原因吗？ 最近刚开始跑目的蛋白的条带，结果发现有很多非特异性条带。 我的样品是从细胞里提取的蛋白，在冰上裂解30min，离心后取上清液，加了上样buffer后煮沸… 显示全部 关注者 28 This methods still works. I would like some real life experiences you have had applying for jobs or a masters program nearby after graduating from wgu, if you dont mind sharing. Although ive read as much research as i can online, its a hit or miss depending on whos talking. Western blot（蛋白质印迹法）是一种用于检测和分析特定蛋白质的实验技术，广泛应用于生物医学研究中。以下是western blot的详细原理、实验步骤、数据处理和作图过程。 一、实验原理 western blot的基本原理可以分为以下几个步骤： 蛋白质分离：通过聚丙烯酰胺凝胶电泳（page）对样品中的蛋白质进行. Most of the time it was a western moose and not the western bull moose -. - anyways i got a perfect western bull moose hunted now thanks to your very precise guide. Some even said their masters program didnt accept wgus degree. Ironic ultranationalistic memes about western european countries (netherlands, luxembourg, germany, france, italy, spain, portugal, greece, uk, ireland, denmark (incl. · thank you very much for sharing. It took me almost 5h to get it but i really had no luck with the moose spawn. Some say wgu was a blessing and some say it was a curse. Some said employers.
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为什么western blot 的条带有很多非特异性条带？ 有可能是一抗的原因吗？ 最近刚开始跑目的蛋白的条带，结果发现有很多非特异性条带。 我的样品是从细胞里提取的蛋白，在冰上裂解30min，离心后取上清液，加了上样buffer后煮沸… 显示全部 关注者 28 This methods still works. I would like some real life experiences you have had applying for...
